A Protein-Interaction Array Inside a Living Cell

A Protein-Interaction Array Inside a Living Cell**

Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell.

We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structural elements of the ER revealed a focal plane (x, y) resolution of <50 nm inside the living cell, c...

Dynamic gene expression profiling using a microfabricated living cell array.

We describe the development of a microfluidic platform for continuous monitoring of gene expression in live cells. This optically transparent microfluidic device integrates high-throughput molecular stimulation with nondestructive monitoring of expression events in individual living cells, hence, a living cell array (LCA). Several concentrations of a soluble molecular stimulus are generated in ...

Living-cell imaging using a photonic crystal nanolaser array.

We proposed and demonstrated a label-free imaging method for living cells using a GaInAsP H0-type photonic crystal nanolaser array. We integrated 441 nanolasers in an arrayed configuration and achieved photopumped lasing with a 100% yield. Then, we attached HeLa cells on it, measured the wavelengths of all nanolasers and used them as pixel information. We acquired cell images, which partially c...

Particle-bubble interaction inside a Hele-Shaw cell.

Hydrodynamic interactions between air bubbles and particles have wide applications in multiphase separation and reaction processes. In the present work, we explore the fundamental mechanism of such complex processes by studying the collision of a single bubble with a fixed solid particle inside a Hele-Shaw cell. Physical experiments show that an air bubble either splits or slides around the par...